RESUMO
BACKGROUND AND OBJECTIVE: Chitosan can be produced through the enzymatic process catalyzed by chitin deacetylase which can be produced by bacteria. The biotransformation of chitin to chitosan by bacteria is interesting because the process is economical and environmentally friendly. This study described the potential of sponge-associated bacterium capability in degrading chitin and forming chitosan. MATERIALS AND METHODS: The bacteria were isolated from sponge Cribrochalina sp. at Manado Bay, Indonesia. In the screening of the chitinase activity of bacteria, chitin media was used. Meanwhile, the transformation of chitin to chitosan was tested by using Chitinase Degrading Activity media. Molecular identification of bacteria was based on 16S rRNA gene sequences. RESULTS: The results showed that the SS1, SS2, SS3, SS4 and SS5 bacterial isolates could degrade chitin based on chitinolytic indexes. These five bacteria could also form chitosan exhibited through the presence of chitosan in the form of precipitation in the fermented broth of bacteria. SS1 had the highest chitinase activity based on the chitinolytic index identified as Bacillus subtilis (100% identity), hence it is called B. subtilis strain SS1. The partial rRNA gene sequences data were deposited at GenBank under accession number MN999892. CONCLUSION: The bacteria strain isolated from Cribrochalina sp. can be utilized in degrading chitin and form chitosan which could be a promising candidate for an economical and eco-friendly process of chitosan.
